LEAPS Meets Life Sciences Conference

Session

Bioimaging

16 May 2023, 17:00

Hotel Hermitage, La Biodola Bay, Elba Island, Italy

176. X-ray structural studies of ba3-type cytochrome c oxidase

Doris Zoric

16/05/2023, 17:00

Poster

Cytochrome c oxidase (CcO) catalyses the reduction of molecular oxygen to water while the energy released in this process is used to pump protons across a biological membrane. Even though many members of the CcO superfamily have been structurally characterized in detail, there is no detailed structural understanding of how unidirectional proton translocation takes place. A billion-fold jump in...

271. Tutorial 4: Advanced X-ray imaging: from the nanoscale at synchrotrons to clinical applications in hospitals

Marco Stampanoni (Paul Scherrer Institute and ETH Zürich)

16/05/2023, 17:15

Oral

In this tutorial I will briefly review the principles of synchrotron radiation, introduce the basics of tomographic microscopy and then showcase some of the latest imaging results, with particular focus on time-resolved experiments. I will then further discuss on how some of the tools originally designed for machine diagnostics purposes have developed into powerful, potentially game-changing...

245. Keynote 4: Multi-scale in situ structural biology in the UK national cryoEM facility eBIC

Peijun Zhang (Diamond Light Source/ University of Oxford)

16/05/2023, 18:45

Oral

The electron Bio-Imaging Centre (eBIC) is a UK national cryoEM facility located at the UK synchrotron Diamond Light Source. eBIC provides UK and international scientists with state-of-the-art experimental equipment, expertise, and training, in the field of cryo-electron microscopy, for single particle analysis, electron tomography, electron diffraction, and correlative microscopy. eBIC also...

187. Human lung virtual histology by multi-scale X-ray phase-contrast computed tomography

Jakob Reichmann (University of Göttingen)

16/05/2023, 19:30

Oral

Propagation-based X-ray phase-contrast tomography (XPCT) offers a unique potential to extend histology and pathohistology by a scalable, isotropic resolution without destructive slicing of the specimen and quantitative density-based contrast.

Here, we use virtual histology based on XPCT to image the human lung at synchrotron radiation endstations (P10, DESY) in different setups as well as...

186. Multilevel X-ray imaging approach to assess the sequential evolution of multi-organ damage in neurodegenerative diseases

Dr Francesca Palermo (Institute of Nanotechnology - CNR)

16/05/2023, 20:00

Oral

The 3D complexity of biological tissues and intricate structural-functional connections call for state-of-the-art X-ray imaging approaches. Unlike other imaging techniques, X-ray phase-contrast tomography (XPCT) offers a highly sensitive 3D imaging approach to investigate different disease-relevant networks at levels ranging from the single cell through to the whole organ. We present here a...