May 14 – 19, 2023
Hotel Hermitage, La Biodola Bay, Elba Island, Italy
Europe/Rome timezone

X-ray structural studies of ba3-type cytochrome c oxidase

May 16, 2023, 5:00 PM
Hotel Hermitage, La Biodola Bay, Elba Island, Italy

Hotel Hermitage, La Biodola Bay, Elba Island, Italy

Biodola Bay 57037 Portoferraio (LI) Isola d’Elba - Italy
Poster Bioimaging


Doris Zoric


Cytochrome c oxidase (CcO) catalyses the reduction of molecular oxygen to water while the energy released in this process is used to pump protons across a biological membrane. Even though many members of the CcO superfamily have been structurally characterized in detail, there is no detailed structural understanding of how unidirectional proton translocation takes place. A billion-fold jump in the peak X-ray brilliance delivered by X-ray free electron laser (XFEL) and the development of serial femtosecond crystallography (SFX) allowed the determination of protein structures at room temperature, opening up the opportunities for time-resolved (TR) experiments in measuring ultrafast reactions in proteins. In this project, we track structural changes at the active site of ba3-type cytochrome c oxidase upon photoinitiated release of oxygen molecule from cobal-based cage compound. Additionally, we use X-ray absorption spectroscopy (XAS) to investigate the detailed atomic structures of the metal co-factors in different redox states.

Select Topic 1 Modern Methods in Structural Biology and Dynamics
Select Topic 2 Strategy of Large Facilities

Primary author

Doris Zoric


Jonatan Johannesson (University of Gothenburg) Emil Sandelin (University of Gothenburg) Adams Vallejos (University of Gothenburg) Arpitha Kabbinale (University of Gothenburg) Swagatha Ghosh (Nagoya University) Peter Dahl (University of Gothenburg) Kajsa Sigfridsson Clauss (Lund University, MAX IV Laboratory) Gisela Bränden (University of Gothenburg) Richard Neutze

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