May 14 – 19, 2023
Hotel Hermitage, La Biodola Bay, Elba Island, Italy
Europe/Rome timezone

Time-resolved serial crystallography studies of conformational changes in cytochrome c oxidase

May 18, 2023, 5:15 PM
Hotel Hermitage, La Biodola Bay, Elba Island, Italy

Hotel Hermitage, La Biodola Bay, Elba Island, Italy

Biodola Bay 57037 Portoferraio (LI) Isola d’Elba - Italy


Richard Neutze


Time-resolved X-ray crystallography allows time-dependent structural changes to be visualized as they evolve within protein crystals and can yield unique structural insights into the course of a biochemical reaction (1). Serial crystallography (2) is now routinely used for time-resolved X-ray diffraction studies of macromolecules at X-ray free electron laser and synchrotron radiation facilities (1). As the field grows, biological reactions that are not naturally light sensitive will be increasingly studied using time-resolved serial crystallography. In this work, a laser-flash was used to release photocaged oxygen and thereby initiate the reduction of oxygen to water in microcrystals of cytochrome c oxidase (3). Our structural results suggest how this reaction may be coupled to gating the uptake of protons from the cytoplasm and to the release of protons to the periplasm. Similar interdisciplinary experiments will allow other biological reactions to become accessible to time-resolved diffraction.

  1. G. Branden, R. Neutze, Advances and challenges in time-resolved macromolecular crystallography. Science 373, eaba0954 (2021).

  2. H. N. Chapman et al., Femtosecond X-ray protein nanocrystallography. Nature 470, 73 (2011).

  3. R. Andersson et al., Serial femtosecond crystallography structure of cytochrome c oxidase at room temperature. Sci Rep 7, 4518 (2017).

Select Topic 1 Modern Methods in Structural Biology and Dynamics
Select Topic 2 Novel Biology

Primary author

Richard Neutze


Jonatan Johannesson (University of Gothenburg) Doris Zoric (University of Gothenburg) Emil Sandelin (University of Gothenburg) Arpitha Kabbinale (University of Gothenburg) Adams Vallejos (University of Gothenburg) Swagatha Ghosh (University of Gothenburg) Carl Johan Wallentin (University of Gothenburg) Gisela Bränden (University of Gothenburg)

Presentation materials