Speaker
Prof.
Frank Watt
(National University of Singapore, Singapore)
Description
Nuclear microscopy has now reached the point where sub-100nm resolutions can be routinely achieved for low current application such as Scanning Transmission Ion Microscopy (1). Since simulations and experimental results have indicated that this resolution is maintained through a whole biological cell, then we now have the potential of imaging the interior of whole cells at unprecedented spatial resolutions (2,3).
This review will concentrate on the work carried out so far on both structural and elemental analysis of biological cells using a focused beam of MeV ions. The talk will include the strengths and weaknesses of nuclear microscopy in this field, and also include a description of competing techniques both for elemental analysis and structural imaging.
(1) Watt, F., Chen, X., Chen, C.-B., Udalagama, C.N.B., Van Kan, J.A., Bettiol, A.A. Whole cell structural imaging at 20 nanometre resolutions using MeV ions, (2013) Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, 306, pp. 6-11.
(2) Chen, X., Chen, C.-B., Udalagama, C.N.B., Ren, M., Fong, K.E., Yung, L.Y.L., Giorgia, P., Bettiol, A.A., Watt, F. High-resolution 3D imaging and quantification of gold nanoparticles in a whole cell using scanning transmission ion microscopy, (2013) Biophysical Journal, 104 (7), pp. 1419-1425.
(3) Udalagama, C., Bettiol, A.A., Watt, F. Stochastic spatial energy deposition profiles for MeV protons and keV electrons, (2009) Physical Review B - Condensed Matter and Materials Physics, 80 (22).
Primary author
Prof.
Frank Watt
(National University of Singapore, Singapore)
Co-authors
Prof.
Andrew Bettiol
(National University of Singapore, Singapore)
Dr
Ce-Belle Chen
(National University of Singapore, Singapore)
Dr
Sudheer Kumar Vanga
(National University of Singapore, Singapore)
Ms
Tao Ye
(National University of Singapore, Singapore)
Mr
Zhaohong Mi
(National University of Singapore, Singapore)
