Speaker
Dr
Adam Sikora
(Institute of Applied Radiation Chemistry, Lodz University of Technology, Lodz, Poland)
Description
It is known that aryl boronates can react with hydrogen peroxide, and hypochlorite with the formation of corresponding phenols. Previously, we have shown that arylboronic acids react rapidly, directly and stoichiometrically with peroxynitrite with the formation of corresponding phenols, as major products
(85-90%). High reactivity toward peroxynitrite makes them promising probes for the detection of peroxynitrite in vitro and in vivo, and a useful tool for the studies of peroxynitrite reactivity.
Here we present the results of the study on the reactivity of boronate fluorogenic probe PC1 toward selected ROS/RNS (e.g. peroxynitrite and peroxynitrate).
The boronate probe PC1 was also used in the kinetic studies on the reactivity of peroxynitrite towards selected salen-manganese complexes EUK. Here we show that there is a strong correlation between reported catalase activity of salen-manganese complexes EUK and the second-order rate constants of their reaction with peroxynitrite.
For better understanding of the factors affecting the oxidation of boronates we also carried out some experiments with model para-substituted aryl boronic acids.
Primary author
Dr
Adam Sikora
(Institute of Applied Radiation Chemistry, Lodz University of Technology, Lodz, Poland)
Co-authors
Andrzej Marcinek
(Institute of Applied Radiation Chemistry, Lodz University of Technology, Lodz, Poland)
Dawid Debski
(Institute of Applied Radiation Chemistry, Lodz University of Technology, Lodz, Poland)
Jan Adamus
(Institute of Applied Radiation Chemistry, Lodz University of Technology, Lodz, Poland)
Karolina Guminska
(Institute of Applied Radiation Chemistry, Lodz University of Technology, Lodz, Poland)
Lukasz Piotrowski
(Institute of Applied Radiation Chemistry, Lodz University of Technology, Lodz, Poland)
Piotr Kwiatkowski
(Department of Chemistry, Warsaw University, Warsaw, Poland)
