Speaker
Mr
Sehwan Shim
(Korea institute of radiological & medical sciences)
Description
Purpose: Gastrointestinal syndrome after high-dose acute radiation exposure (GI-ARS) is life-threatening problem. In this study, we examined the radiation mitigation effects of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSC) on intestinal damage of gamma-irradiated rats.
Methods: to investigate the effect of hUCB-MSC on mitigation of intestinal injury following gamma-irradiation, animals were divided into three group; non-irradiated control group, non-treated group and hUCB-MSC transplanted group after irradiation. The animals were exposed 14 Gy in abdomen and sacrificed at 4 days and 8 days after irradiation to examine the various pathological changes related to intestinal injury. hUCB-MSC (1x107cells/animal) was intravenously injected at 4 hours after irradiation.
Result: All animals showed severe diarrhea and piloerection after irradiation. We found histopathological mechanisms that radiation-induced intestinal injury was characterized by remarkable shortened villi, decreased crypt, edema of neural plexus and cellular degradation of ganglions. hUCB-MSC transplanted group was significantly increased the proliferation activity of ileac crypt, compared with irradiation controls. The hUCB-MSC transplantation reduced irradiated intestinal morphological changes such as crypt size and villus height. In addition, the administration of hUCB-MSC increased the expression of platelet derived growth factor receptor-α (PDGFR-α), involved in villus morphogenesis and ubiquitin c-terminal hydrolase-l1 (UCH-L1), a marker of neuronal cells. These results suggest that hUCB-MSC has the protective effects not only on the intestinal cells but also on the neuronal cells in the small intestine.
Conclusion: Although further study will be needed about time point, route of injection or combined treatment with other drugs, our results suggest that hUCB-MSC administration is useful for regeneration of intestinal cell and reconstruction of organic structure against radiation-induced GI syndrome.
Primary author
Mr
Sehwan Shim
(Korea institute of radiological & medical sciences)
Co-authors
Mr
Jong-geol Lee
(Korea institute of radiological & medical sciences)
Dr
Seung-Sook Lee
(Korea institute of radiological & medical sciences)
Ms
Sun-Joo Lee
(Korea institute of radiological & medical sciences)
Dr
Sunhoo Park
(Korea institute of radiological & medical sciences)
Mr
Won-Suk Jang
(Korea institute of radiological & medical sciences)
