Speaker
Mindaugas Malcius
(Center for Physical Sciences and Technology, Vilnius, Lithuania)
Description
In this investigation pancreatic cancer cells MIA PaCa-2 were irradiated with various doses
of proton radiation: from 1 Gy to 30 Gy. Experiments were done using Tandetron 4110 accelerator
in Center for Physical Sciences and Technology(which is in Vilnius, Lithuania). Irradiation was
executed when cells formed monolayer. Distance from exit window to sample was 22 mm.
Diameter of proton exit window was 0.175 mm, window was cover with 5 um Mylar film.
Properties of proton beam was evaluated with GEANT 4 (energy losses in Mylar and air, range in
matter, processes of interaction, spectra of electrons created by protons etc.) and MCNPX (beam
radius dependence on distance from proton exit (to the atmosphere) to the sample. Initial energy of
protons were 1.6 MeV, beam radius (FWHM) at sample plane was 0.85 mm. It is important to know
beam radius to assure even dose distribution.
It was calculated number of protons and created electrons per cell and per DNA volume. From
modeling results (processes of interaction) possible number of lesions in DNA was evaluated. From
these numbers presumptive number of DSB's per cell could be calculated. Actual number of DSB's
was checked using flow cytometer.
Survival using colony method was also measured for dose control.
Primary author
Mindaugas Malcius
(Center for Physical Sciences and Technology, Vilnius, Lithuania)
Co-authors
Dr
Artūras Plukis
(Center for Physical Sciences and Technology, Vilnius, Lithuania)
Aušra Liubavičiūtė
(Center for Physical Sciences and Technology, Vilnius, Lithuania)
Mindaugas Gaspariūnas
(Center for Physical Sciences and Technology, Vilnius, Lithuania)
Dr
Vidmantas Remeikis
(Center for Physical Sciences and Technology, Vilnius, Lithuania)
Dr
Vitalij Kovalevskij
(Center for Physical Sciences and Technology, Vilnius, Lithuania)
