EUROPEAN RADIATION RESEARCH 2012

Session

Poster Session 1

16 Oct 2012, 16:00

Vietri sul Mare

49. Steady-state and pulse radiolysis study of selected alkaloids in aqueous DNA and human serum albumin (HSA) systems

Mrs Milena Marszalek (Technical University of Lodz)

16/10/2012, 16:00

Early Biophysical Processes

poster preferred

Isoquinoline alkaloids widely coexist in many Chinese herbal medicines, e.g. Rhizoma Coptidis and Caulis Mahoniae. They possess antimicrobial, antidiarrheal and cardiovascular activities, and recently have been also suggested as potential drugs for treatment of the Alzheimer disease and as photo- or radiosensitizers in cancer therapy [1]. Small molecules bind to the DNA and HSA in different...

71. C-centered paramagnetic species generated radiolytically in molecular sieves.

Mr Marcin Sterniczuk (Institiute of Nuclear Chemistry and Technology)

16/10/2012, 16:01

Other

poster preferred

Radicals play an important role in many radiolytic and catalytic reactions. However, their structure and properties are difficult to study because of extremely high reactivity. Matrix isolation technique in noble gas matrices and trapping in zeolites are the methods of choice for studying electronic structure and geometry of radical ions in solids. We present the study of paramagnetic carbon...

115. ABSOLUTE CROSS SECTIONS FOR THE FRAGMENTATION OF BIOLOGICALY RELEVANT MOLECULES AFTER IONISATION

Mr Alexander Arndt (Physikalisch-Technische Bundesanstalt (PTB))

16/10/2012, 16:02

Early Biophysical Processes

poster preferred

The advent of heavy ions for cancer therapy has emphasised the need for novel dosimetric concepts that ideally should also account for the biological effectiveness of radiation. One approach to obtain such new dosimetric concepts is the field of nanodosimetry, where the particle track structure is taken into account by measurement [1] or by simulation [2] of ionisation cluster size...

142. Radiolytic study of interactions of organic cations with heparin

Malgorzata Jakubowska (Institute of Applied Radiation Chemistry, Lodz University of Technology, Lodz, Poland)

16/10/2012, 16:03

Other

poster preferred

Glycosaminoglycans are linear polysaccharides consisting of repeating disaccharide units. Because of the presence of negatively charged sulfate and carboxyl groups they are polyelectrolytes. They occur on animal cell surface and in the extracellular matrix. Glycosaminoglycans present on endothelium cell surface allow an interaction with the underlying subendothelium and blood components, but...

164. Radiolysis and photolysis of coralyne and sanguinarine water solutions.

Ms Anna Konarska (Institute of Applied Radiation Chemistry, Lodz University of Technology)

16/10/2012, 16:04

Other

poster preferred

Sanguinarine and coralyne are isoquinoline alkaloids isolated from plants. The use of these alkaloids as medicines by humans dates to several thousands of years ago and recently they are applied in cancer therapy. Naturally occurring alkaloids are effective in cancer treatment but its mechanism of action has not been fully elucidated. Sanguinarine is reduced upon entering the cell and then...

225. Reactivity of hydroethidine towards peroxyl radicals

Mr Bartosz Michalowski (Institute od Applied Radiation Chemistry, Lodz University of Technology, Lodz, Poland)

16/10/2012, 16:05

Other

poster preferred

Because of the fact that the oxidation product of hydroethidine (HE), 2-hydroxyethidium (2-OH-E+), is an unique, fluorescent marker for superoxide radical anion (O2•-), hydroethidine has become one of the most used fluorogenic probes for its intracellular detection. Although, HE can be used rather as a qualitative than quantitative probe for detection of superoxide, it is still worthwhile to...

72. CytoBayesJ: Software tools for Bayesian analysis of cytogenetic radiation biodosimetry data

Dr Elizabeth Ainsbury (Health Protection Agency)

16/10/2012, 16:06

Biological and Physical Dosimetry

poster preferred

The classical methods for dose estimation in radiation cytogenetics are now extremely well defined. However, classical statistical methodology requires that chromosome aberration yields are considered as something “fixed.” This 'frequentist' approach takes into account only the chromosomal damage which has been identified in a particular sample, and as such does not fully consider the...

73. RENEB – Realizing the European Network of Biological Dosimetry

Dr Ulrike Kulka (German Federal Office for Radiation Protection)

16/10/2012, 16:07

Biological and Physical Dosimetry

poster preferred

Creating a sustainable network in biological and retrospective dosimetry that involves a large number of experienced laboratories throughout the European Union (EU) will significantly improve the accident and emergency response capabilities in case of a large-scale radiological emergency. A well organised cooperative action involving EU laboratories will offer the only chance for a fast and...

77. Intra- and inter-individual variability of the dicentrics frequency in human lymphocytes exposed to ionizing radiation at different temperatures

Prof. Anna Lankoff (Institute of Nuclear Chemistry and Technology, Centre for Radiobiology and Biological Dosimetry, Warsaw, Poland; Jan Kochanowski University, Dept. of Radiobiology and Immunology, Kielce, Poland)

16/10/2012, 16:08

Biological and Physical Dosimetry

poster preferred

Dicentric assay is currently a gold standard for classic biodosimetry because it possess all features of an ideal assay: low background level, clear dose-effect relationship for different radiation qualities and dose rates, specificity to ionizing radiation, low invasiveness, good reproducibility and comparability of in vitro and in vivo results. This method is based on the assumption that the...

78. Dose-response curves for Portuguese population: Chromosomal aberrations and Micronuclei assays

Dr Octávia Monteiro Gil (Instituto Tecnológico e Nuclear, Instituto Superior Técnico, Universidade Técnica de Lisboa)

16/10/2012, 16:09

Biological and Physical Dosimetry

poster preferred

The main aim of a radiation study is to assess the risk of the population exposed to ionizing radiation. The work of the dose assessment may be very difficult because dose information about the population exposed is often indirect and incomplete. It is very important, therefore, to find a way to estimate reasonable and reliable doses of the population by a retrospective method such as...

79. Influence of image acquisition and analysis parameters on γ-H2AX dose-response curves in human lymphocytes exposed to ionizing radiation

Dr Maria Wojewódzka (Institute of Nuclear Chemistry and Technology, Centre for Radiobiology and Biological Dosimetry, Warsaw, Poland)

16/10/2012, 16:10

Biological and Physical Dosimetry

poster preferred

The scoring of γ-H2AX foci as a measure for double-strand breaks (DSB) can be used to estimate exposure to the very low doses of radiation and shows that the yield of foci induced by ionizing radiation increases linearly with the radiation dose. These findings indicate that quantitation of γ-H2AX foci is a promising tool for biological dosimetry. Quantification of γ-H2AX foci can be achieved...

83. A novel analytical method of biological treatment plan optimization

Dr Francisco Cutanda Henríquez (Instituto de Salud Carlos III)

16/10/2012, 16:11

Biological and Physical Dosimetry

poster preferred

Modern treatment planning allows the achievement of complex goals. Minimum and maximum doses cannot be considered as adequate figures of merit for the quality of a plan, and that is the reason why a complex set of parameters has to be evaluated when an irradiation plan is to be assessed. Particularly, radiobiological indices are useful tools for this evaluation, as they relate the plan to...

88. Establishment of an 192 Ir γ-ray calibration curve for lymphocyte dicentric assay in case of occupational or accidental exposure in industrial radiography

Dr Katarzyna Golnik (Central Laboratory for Radiological Protection)

16/10/2012, 16:12

Biological and Physical Dosimetry

poster preferred

The analysis of dicentric chromosomes in human peripheral blood lymphocytes is the most reliable and accurate method for estimating a recent absorbed dose following external exposure to ionising radiation. The frequencies of dicentrics per grey vary depending on the variations in both the linear energy transfer (LET) and the relative biological effectiveness (RBE) of any particular radiation....

92. Sodium tartrate as ESR dosimetric material for low-dose measurement

Dr Hasan TUNER (Department of Physics, Faculty of Science and Literature, University of Balikesir, Balikesir, Turkey)

16/10/2012, 16:13

Biological and Physical Dosimetry

poster preferred

Potential ESR dosimetric application of different compounds of sodium tartrate such as sodium tartrate dihydrate (NaTA), sodium bitartrate monohydrate (Na-bTA), and potassium sodium tartrate tetrahydrate (KNaTA) were investigated in the range of 0.5 – 50 Gy. Comparisons were also made with alanine (AL) [1], ammonium tartrate (AmTA) [2] and lithium formate (LiF) [3] samples. It is found that...

94. GAMMA DOSE RATE AND EFFECTIVE INDOOR GAMMA DOSE IN DWELLING OF CAMPANIA REGION

Dr Mariagabriella Pugliese (Dipartimento di Scienze Fisiche, Università degli Studi di Napoli Federico II, Napoli, Italy and INFN Sezione di Napoli)

16/10/2012, 16:14

Biological and Physical Dosimetry

poster preferred

Ionizing radiation from natural sources such as cosmic rays and terrestrial radiation, is the major contribute to the exposure for public population. In particular gamma radiation is the largest contributor to the external dose of the humans. Today it is assessed that the majority of inhabitants in urban areas spends about 80% of their time indoor, so their exposure to ionizing radiation from...

97. Cytogenetic Assays For Assessment Of Ionizing Radiation-Induced DNA Damage

Ms najmeh assadi (babol university of medical sciences)

16/10/2012, 16:15

Biological and Physical Dosimetry

poster preferred

During humans lifetime,they are exposed to physical, chemical, and biological agents. Ionizing radiation as a ubiquitous environmental physical agent induces DNA damage directly (as a result of deposition of energy in cells) or indirectly (as a result of free radical formation and oxidative damage . And because of Radiation usage in the treatment of a wide range of malignancies. Therefore,...

98. Preservation of chestnut fruits by gamma irradiation: inter-comparison of absorbed dose results using three types of dosimeters

Dr Amilcar Lopes Antonio (1-School of Agriculture, Polytechnic Institute of Bragança, Portugal. 2-Department of Fundamental Physics, University of Salamanca, Spain. 3-IST/ITN, Nuclear and Technological Institute, Portugal)

16/10/2012, 16:16

Biological and Physical Dosimetry

poster preferred

Food irradiation is a well established process, approved by international organizations of food (FAO – Food and Agriculture Organization) and health (WHO – World Health Organization), and regulated in European Union by the Directive 1999/2/EC. Chestnuts are a seasonal fruit that must be post-harvest treated to meet the food safety regulations. Until recently, this was done with methyl bromide...

118. Evolution of the Canadian Biological Dosimetry Network

Dr Ruth Wilkins (Health Canada)

16/10/2012, 16:17

Biological and Physical Dosimetry

poster preferred

In the event of a large scale radiological/nuclear emergency, biological dosimetry is essential for providing timely assessments of radiation exposure for the general population and to identify first responders who must be restricted from further exposure. The dicentric chromosome assay (DCA) is currently the accepted biodosimetry method for radiation dose assessment, however in a mass...

128. Dose assessment and consideration in the deep place of the skin at the time of liniment use

Mr Ryo Akima (Department of Diagnostic Radiology & Radiation Oncology, Kochi Medical School)

16/10/2012, 16:18

Biological and Physical Dosimetry

poster preferred

Purpose: For patients who experience radiation dermatitis during radiotherapy, we sometimes use pharmaceutical agents applied topically, such as creams and lotions. Irradiation of treated parts thus sometimes occurs. If a substance exists in the skin surface, the skin dose might be slightly affected by such exposure, and the agent could become a factor associated with radiation injury. The...

138. ASSESSMENT OF THE FREQUENCY OF CHROMOSOMAL ABERRATIONS IN MAYAK WORKERS EXPOSED OCCUPATIONALLY

Mrs Natalia Sotnik (Southern Urals Biophysics Institute)

16/10/2012, 16:19

Biological and Physical Dosimetry

poster preferred

Objective: Assessment of structural genome damages in Mayak workers exposed occupationally to prolonged external gamma-radiation and/or internal alpha-radiation using mFISH. Material and Methods: Slides of 64 workers were analyzed in the study. Average age of the included individuals was 73.3±0.9 years. Workers included in the study were exposed occupationally to prolonged external...

139. Cytogenetic effects of chronic exposure of the Red Bone Marrow in humans

Ms Alexandra Vozilova (Russian Federation)

16/10/2012, 16:20

Biological and Physical Dosimetry

poster preferred

Population exposure to ionizing radiation in the Urals region (Russia) occurred as a result of failures in the technological processes at the Mayak plutonium facility in early 1950s. A major source of environmental contamination was releases of liquid radioactive wastes into the Techa River in 1949–1956 with peak amounts from September 1950 until November 1951. Over 30,000 persons living in...

141. THRESHOLD LIMITS IN CYTOGENETIC INDICATION AND BIODOSIMETRY IN TERMS OF CHRONIC EXPOSURE

Dr Sergey Osovets (Southern Urals Biophysics Institute)

16/10/2012, 16:21

Biological and Physical Dosimetry

poster preferred

In spite of the great variety of bioindication and biodosimetry methods, World Health Organisation (WHO) and International Atomic Energy Agency (IAEA) accept dose reconstructions by cytogenetic methods based on the analysis of specific chromosomal aberrations (DIC, FISH techniques) on metaphases from peripheral blood cells. Capabilities and characteristics of cytogenetic indication and...

160. NK cells and their response to ionizing irradiation

Ms Lenka Zárybnická (Faculty of Military health Sciences, University of Defence)

16/10/2012, 16:22

Biological and Physical Dosimetry

poster preferred

Natural killer cells (NK cells) belong to white blood cells which care for anti-tumour and anti-viral control in organism. They can be distinguished from other lymphocyte subpopulations by specific surface markers detection. Our study is aimed at a sensitivity of NK cells to ionizing gamma irradiation. Generally, lymphocytes induce cell apoptosis in a consequence of gamma irradiation....

162. Gene Expression Analysis in Human Peripheral Blood Lymphocytes for Biodosimetric Applications after Low and High Dose Gamma-Irradiation

Dr Ralf Kriehuber (Radiation Biology Group, Department of Safety and Radiation Protection, Forschungszentrum Jülich, D-52425 Jülich, Germany)

16/10/2012, 16:23

Biological and Physical Dosimetry

poster preferred

In case of a large-scale radiation accident with involvement of individuals without physical dosimeters it is important to identify individuals who have received a moderate to high radiation dose to ensure proper medical care. As current methods are time-consuming, a fast and reliable method based on gene expression alterations is developed. Human blood of 3 male and 3 female healthy donors,...

175. A metaphase finder using single manufacturer's product

Dr Akira Furukawa (National Institute of Radiological Sciences (Japan))

16/10/2012, 16:24

Biological and Physical Dosimetry

poster preferred

Metaphase finder is a tool for biodosimetry that finds metaphase chromosomes among blood cells on slide glasses. It consists of microscope, auto-focus system, automated X-Y stage, camera and computer. It does the image diagnosis of the microscopic images of the slide glasses, and displays the positions of metaphase cells. A metaphase finder is used for the personnel worked at Fukushima nuclear...

216. Radiation dose estimative using computational murine model from Gd-159 nanostructured radiopharmaceutical

Dr ADRIANO M. SANTOS (Nuclear Technology Development Center - National Commission on Nuclear Energy)

16/10/2012, 16:25

Biological and Physical Dosimetry

poster preferred

Different therapeutic radiopharmaceuticals containing the P-32, Re-188, Sr-89, and Y-90 radioisotopes have been used effectively in alleviating bone pain resulting from metastases, synovectomy, and tumors. These radioisotopes all present the ability to emit high doses of beta radiation, leading to the death of tumor cells. Similarly, the Gd-159 isotope emits negative beta (1001 keV) and gamma...

224. Development of nuclear safety and radiological protection methods for the nuclear power engineering’s current and future needs.

Dr Kamil Brzoska (Institute of Nuclear Chemistry and Technology, Warsaw, Poland)

16/10/2012, 16:26

Biological and Physical Dosimetry

poster preferred

The strategic research project entitled “Technologies Supporting Development of Safe Nuclear Power Engineering” is a response to the demand for the Poland’s energetic safety optimization in the light of nuclear power engineering introduction. The strategic project will link research performed by Polish research teams with that which is carried out abroad and provide Polish scientists and...

240. Retrospective dose assessment by EPR and OSL in mobile phones

Dr Paola Fattibene (Department of Technology and Health, Istituto Superiore di Sanità, Rome, Italy)

16/10/2012, 16:27

Biological and Physical Dosimetry

poster preferred

In the retrospective dose assessment of individuals potentially exposed to ionizing radiation after an accident, dosimetry with inert materials can complement or be used as an alternative to biodosimetry assays. In the framework of the EU funded project Multibiodose (www.MULTIBIODOSE.eu), it is proposed to implement a combined system of dose assessment based on the use of EPR and OSL...

259. Estimation of entrance surface dose and compliance with diagnostic reference levels for selected plane film X-ray radiographic procedures in Poland

Dr Katarzyna Majchrzak (National Centre for Radiation Protection in Health Care, Lodz, Poland)

16/10/2012, 16:28

Biological and Physical Dosimetry

poster preferred

Introduction: In 2011 National Centre for Radiation Protection in Health Care (NCRPHC) together with sanitary inspections accomplished the pilot project for estimation of the entrance surface dose (ESD) for the most frequent plane film X-ray examinations. As a result the compliance with diagnostic reference levels (DRLs) in routine practice of the supervised departments were...

19. Measures of DNA damage sensitivity correlate bladder cancer cell treatment sensitivity in vitro and outcome in vivo.

G Jones (University of Leicester)

16/10/2012, 16:29

DNA Damage and Repair

poster preferred

Bladder cancer patients suffer significant rates of treatment failure, including high rates of recurrence and poor outcomes for advanced disease. If mechanisms to improve tumour cell treatment sensitivity could be identified and/or if patient tumour response could be predicted, it should be possible to improve both control and survival rates, i.e. by selecting the most appropriate treatment...

50. Estimation of transgenerational effects of radiation in male germ cells of mice using high-density microarray CGH platform

Dr Jun-ichi Asakawa (Dept. of Genetics, Radiation Effects Research Foundation)

16/10/2012, 16:30

Other

poster preferred

We used two methods to estimate genetic effects of radiation. The first method was the comparative genomic hybridization (CGH) with high-density microarray containing 2.1 million probes (HD-2 microarray), which permits high-resolution examination of copy number changes (CNV: deletions and amplifications) throughout the whole genomes between two DNA samples. Refinement of the experimental and...

54. Analyses of CT induced DNA damage to determine radiation sensitivity of different age groups, especially young children – a pilot study in the frame of the EU EPI – CT project

Mrs Ursula Oestreicher (Bundesamt für Strahlenschutz)

16/10/2012, 16:31

DNA Damage and Repair

poster preferred

The increasing use of paediatric computed tomography (CT) worldwide has raised the question of possible late effects from exposure to ionising radiation. The European collaborative EPI-CT project aims at studying the cancer risks and the underlying biological effects in an international cohort study. The project is coordinated by the Section of Environment and Radiation at the International...

58. TOPORS modulates H2AX discriminating genotoxic stresses

Dr CHA SOON KIM (Radiation Health Research Institute, KHNP)

16/10/2012, 16:32

DNA Damage and Repair

poster preferred

H2AX plays an important role in chromatin reorganization implicated in DNA repair and apoptosis under various DNA damaging conditions. In this study, the interaction between TOPORS and H2AX was verified in vitro and in vivo using mammalian cell extracts exposed to diverse DNA damaging stresses such as ionizing radiation, doxorubicin, camptothecin, and hydrogen peroxide. In vitro assays for...

87. Differences in correct and incorrect rejoining of DNA double-strand breaks in human fibroblasts having different radiosensitivity

Dr Ghazi Alsbeih (King Faisal Specialist Hospital & Research Centre)

16/10/2012, 16:33

DNA Damage and Repair

poster preferred

DNA double-strand breaks (DSBs) in irradiated cells are primarily responsible for the biological effects of ionizing radiation. The restitution of the broken ends depends on repair potential of the cells and determines their fate: survival, transformation or death. It has been suggested that differences in DSBs repair capacity between individuals and cultured cell strains may explain the...

103. Super resolution microscopy of ion induced repair foci in human HeLa cells

Judith Seel (Universität der Bundeswehr München)

16/10/2012, 16:34

DNA Damage and Repair

poster preferred

High LET irradiation of living cells using heavy ions generates a high amount of DNA double strand breaks (DSB) in close vicinity to each other along the ion track. Various repair proteins cluster to the damage sites, e.g. γH2AX and 53BP1, and form repair foci of a gross size of about 1 µm. Due to the fact that one focus covers more than one DSB, a fine structure within the focus can be...

119. The study of DNA damage in lymphocytes and granulocytes after whole body irradiation in mice

Zuzana Sinkorova (University of Defence)

16/10/2012, 16:35

DNA Damage and Repair

poster preferred

Whole body ionizing gamma irradiation represents genotoxic stress, which has a decisive influence on all blood cell lines. Lymphocytes belong to the most radiosensitive cell lineages. After exposure of the organism to ionizing irradiation they die by apoptosis, the extent of which depends on the reeived dose. Bone marrow contains a reserve pool of functionally mature granulocytes, which might...

140. Application of FISH technique to study chromosome 1, 2 and 4 in colorectal cancer patients

Dr Justyna Miszczyk (Institute of Nuclear Physics PAN, Kraków, Poland)

16/10/2012, 16:36

Biological and Physical Dosimetry

poster preferred

Colorectal cancer is one of the most commonly occurring malignancies in both men and women. However, application of radiotherapy might be beneficial depending on individual susceptibility or even may cause risk of secondary cancers and others complications. Therefore, studies on influence of endogenic or egzogenic factors on the body’s response in modeling a therapeutic procedure are still...

156. DNA end resection is required for the repair of complex lesions in human G1 cells

Nicole Averbeck (GSI Helmholtzzentrum für Schwerionenforschung)

16/10/2012, 16:37

DNA Damage and Repair

poster preferred

We study DNA double strand break (DSB) repair with heavy ion radiation. As densely ionizing radiation it generates strictly localized DSBs within the nucleus and thus, represents an excellent tool for investigating the recruitment of repair factors to DSBs by immunofluorescence. Applying this technique we were able to show that with increasing lesion complexity DSBs induced in the G1 cell...

157. Single and double strand breaks of DNA in peripheral blood lymphocytes of chronically exposed individuals

Ms Alyona Pogodina (Ural Research Centre for Radiation Medicine)

16/10/2012, 16:38

DNA Damage and Repair

poster preferred

Our purpose was investigation DNA single-strand breaks (SSB) and double-strand breaks (DSB) levels in peripheral blood lymphocytes exposed individuals 60 years after exposure. Two groups of humans was formed: exposed (51 subjects) and non-exposed (24 subjects). In the group of exposed individuals were identified individuals with chronicradiation syndrome (CRS) – 8 individuals, and with...

182. Ionizing radiation induced genomic instability in human fibroblasts and modulating effects of telomerase activities in different processes of DNA DSB repair

Dr Otilia Nuta (Radiation and Environmental Science Centre, DIT, Republic of Ireland; Department of Toxicogenetics, Leiden University Medical Center, the Netherlands; Health Protection Agency, Centre for Radiation, Chemical and Environmental Hazards, Oxfordshire, U.K.)

16/10/2012, 16:39

DNA Damage and Repair

poster preferred

There have been suggestions that the telomerase system is involved in radiation induced genomic instability. The aim of this study was to investigate the involvement of telomerase in the development of chromosomal damage and its repair at different stages of the cell cycle following an exposure of human fibroblasts to ionizing radiation. We examine the relationship between several...

190. PROTON RADIATION DOSE INFLUENCE ON NUMBER OF DSB'S IN CANCER CELLS

Mindaugas Malcius (Center for Physical Sciences and Technology, Vilnius, Lithuania)

16/10/2012, 16:40

DNA Damage and Repair

poster preferred

In this investigation pancreatic cancer cells MIA PaCa-2 were irradiated with various doses of proton radiation: from 1 Gy to 30 Gy. Experiments were done using Tandetron 4110 accelerator in Center for Physical Sciences and Technology(which is in Vilnius, Lithuania). Irradiation was executed when cells formed monolayer. Distance from exit window to sample was 22 mm. Diameter of proton exit...

205. The influence of end resection by CtIP on the Artemis dependent DNA double-strand break repair pathway in G1 and G2 phase after high- and low-LET irradiation

Dr Sandro Conrad (Technical University of Darmstadt)

16/10/2012, 16:41

DNA Damage and Repair

poster preferred

The major repair pathways for DNA double-strand breaks (DSBs) are non-homologous end-joining (NHEJ) and homologous recombination (HR). NHEJ is active in all cell cycle phases and repairs DSBs with fast kinetics. It represents the major repair pathway in G1 and G2. In contrast, HR is the main repair pathway for replication-associated DSBs in S phase and also repairs a subfraction of DSBs in G2....

222. Accumulation of non-DSB oxidative clustered DNA lesions in irradiated BRCA1 deficient cells affects mitigation of radiotoxicity and enhances chromosomal instability

Dr Georgia Terzoudi (Radiobiology Laboratory, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, National Centre for Scientific Research “Demokritos” Athens, Greece)

16/10/2012, 16:42

DNA Damage and Repair

poster preferred

Radiation can increase significantly the level of oxidative stress that cell have to sustain and mitigate. Persistent of such stress can lead to unrepaired or misrepaired DNA lesions the accumulation of which is considered for many the initiation point for chromosomal instability, neoplastic transformation and human pathogenesis. Indeed, the presence of high oxidative stress in human patients...

223. Involvement of chromatin organization at different cell-cycle stages in the conversion of DNA lesions into chromatid breaks and exchanges

Dr Gabriel Pantelias (Radiobiology Laboratory, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, National Centre for Scientific Research “Demokritos” Athens, Greece)

16/10/2012, 16:43

DNA Damage and Repair

poster preferred

The formation of diverse chromosomal aberrations following irradiation at different cell-cycle stages remain a long standing controversy, probably because most of the studies have focused on elucidating the enzymatic repair mechanisms involved using simple DNA substrates. Yet, recognition, processing and repair of DNA damage occur within the nucleoprotein complex of chromatin which is dynamic...