Speaker
Ms
Węgierek-Ciuk Aneta
(Dept. of Radiobiology and Immunology, Jan Kochanowski University, Kielce, Poland)
Description
Several observations indicate that normal tissue hypersensitivity may be related to certain genetic factors. In view of the importance of DNA repair in cell and tissue response to radiation, single nucleotide polymorphisms (SNPs) in the genes responsible for DNA damage signaling and repair pathways seem to be suitable candidates in the search for the genetic basis of clinical normal tissue radiosensitivity. The aim of our study was to investigate whether SNPs selected in XRCC1, XRCC3 and OGG1 genes are associated with an increased risk of adverse reactions to radiotherapy in patients treated for gynecologic and prostate cancers. Furthermore, the reliability of radiation-induced apoptosis in the prediction of acute reactions within the studied population was investigated. The study population consisted of 30 women with cervical and uterine cancer and 30 man with prostate cancer. All woman received a tumor dose of 50,4 Gy in 28 fractions and all man received a tumor dose of 74 Gy in 37 fractions. Acute normal tissue reactions were scored according to RTOG criteria. Peripheral blood from cancer patients was collected before radiotherapy and irradiated in vitro with a dose of 2 Gy. The frequency of apoptotic cells was determined by flow cytometer after FITC-Annexin-V staining. Polymorphic sites in XRCC1 (194Arg/Trp, 399Arg/Gln), XRCC3 (IVS5-14), and OGG1 (326Ser/Cys) were examined by (PCR)-restriction length fragment polymorphism. The results of the present study showed that the only XRCC1 codon 194 variant showed a significant protective effect in prostate cancer patients (p = 0.006). Gynecological cancer patients with low frequency of radiation-induced apoptosis showed a greater grade of complications than patients with high frequency of radiation-induced apoptosis (p = 0,031).
This work was supported by stipendium to Aneta Węgierek-Ciuk"Wiedza i Gospodarka - rozwój kompetencji naukowych i biznesowych dla wzrostu konkurencyjności gospodarki regionalnej"współfinansowanego ze środków Europejskiego Funduszu Społecznego.
Primary author
Ms
Węgierek-Ciuk Aneta
(Dept. of Radiobiology and Immunology, Jan Kochanowski University, Kielce, Poland)
Co-authors
Dr
Agnieszka Florek
(Cancer Centre, Kielce, Poland)
Prof.
Andrzej Wojcik
(Dept. of Radiobiology and Immunology, Jan Kochanowski University, Kielce, Poland;GMT Dept., Stockholm University, Sweden)
Anna Lankoff
(Dept. of Radiobiology and Immunology, Jan Kochanowski University, Kielce, Poland; Centre for Radiobiology and Biological Dosimetry, Institute of Nuclear Chemistry and Technology, Warsaw, Poland)
Dr
Halina Lisowska
(Dept. of Radiobiology and Immunology, Jan Kochanowski University, Kielce, Poland)
Dr
Michal Arabski
(Dept. of Microbiology, Jan Kochanowski University, Kielce, Poland)
Dr
Piotr Kedzierawski
(Cancer Centre, Kielce, Poland)
Dr
Stanislaw Gozdz
(Cancer Centre, Kielce, Poland)
