Speaker
Ms
Alla Kubashko
(Research Center for Radiation Medicine)
Description
Increase of chronic diseases number among Chornobyl Nuclear Power Plant (ChNPP) accident clean-up workers require more detailed study of mechanisms forming pathological background for such clinical implications. The prolonged disturbances of oxidative homeostasis (OH) on the top of radiation induced molecular-genetic disorders can be a ponderable factor for morphofunctional changes which afterward might realize into chronic clinical manifestations. Thus, investigation of OH status in irradiated persons allows understanding the character of biochemical changes in remote period depending on absorbed doses.
ОH status was studied in 355 males clean-up workers aged (48.62±0.55) who was irradiated in 1986-1988 in dose range (1.22±0.06) Gy during elimination of ChNPP accident aftermath. The investigation term (1992-2009) was divided on three periods: I -1992-1995, II - 1996-1999, III - 2006-2009. The controls were formed by 177 practically healthy men without radiation contact in accordance to each period. The levels of lipoperoxidation products (LPP) - phospholipids with isolate, double and triple bonds, tiobarbiture acid reactive species (TBARS), сarbonyl proteins (CP), reduced glutathione (GSH), erythrocyte superoxidedismutase (Zn/CuSOD), catalase (E-CAT), glutathione peroxidase (GPx) and activities are determined in peripheral blood samples. The integral factor of antioxidant status (FAOS) for estimation of ОH derangement was calculated as well.
The disturbance of OH in all examinees was observed during the whole investigation term. It was revealed that in persons irradiated in dose >0.25 Gy the integral FAOS level dropped by 1.52 times (p=0.001) since I to III period against the background of LPP accumulation with double, triple bonds, TBARS, CP, GSH levels and decreased activity of E-CAT, switching over of antioxidant protection to GPx and while the decline of Zn/CuSOD activity was marked only in persons with absorbed doses >2.00 Gy.
The LPP and CP content, Zn/CuSOD activity and FAOS level dynamic of changes correlated with absorbed doses and did not connect with age factor in examined persons during investigation term.
Thus, the enhancement of OH disturbances in clean-up worker of ChNPP accident as postaccidental terms increased, peculiarities of which correlated with absorbed dose, possibly is a result of individualized sensitivity to irradiation that forms the pathobiochemical background for development and maintenance of steady clinical implications
Primary author
Prof.
Ludmyla Ovsyannikova
(Research Center for Radiation Medicine)
Co-authors
Ms
Alla Kubashko
(Research Center for Radiation Medicine)
Prof.
Anatoly Chumak
(Research Center for Radiation Medicine)
Mrs
Svetlana Alekhina
(Research Center for Radiation Medicine)
