Speaker
Description
Volumetric imaging of mm-sized soft tissue samples with micron resolution opens new possibilities both in clinical and research settings, driven by the growing need for studying micro and nanoscale structures in a three-dimensional context at the mesoscale.
In this talk I will report the first proof of concept for three-dimensional soft tissue imaging with a laboratory-based x-ray microscope based on intensity-modulation masks, allowing for multi-modal retrieval of transmission, refraction, and scattering. The microscope allows for micron resolution in the resolved channels (transmission and refraction), while it can reach the nanoscale in the scattering channel. The combination of micron-level spatial resolution, set by the use of intensity modulation masks, and the enhanced contrast, arising from phase-based imaging, allows for the. Additionally, the high-content nature of this technique allows for the correlation of the scattering signal with nanoscale structures found in tissue, namely cellular nuclei and extra-cellular matrix.
This talk will address the challenges of correlating microscale cellular visualisation with the broader context of tissue architecture at the mesoscale, using a multi-scale imaging approach. Finally, I will discuss the potential of integrating X-ray microscopy with in-operando mesoscale imaging to investigate fluid dynamics processes within tissue.
