Mrs
Milena Marszalek
(Technical University of Lodz)
16/10/2012, 16:00
Early Biophysical Processes
poster preferred
Isoquinoline alkaloids widely coexist in many Chinese herbal medicines, e.g. Rhizoma Coptidis and Caulis Mahoniae. They possess antimicrobial, antidiarrheal and cardiovascular activities, and recently have been also suggested as potential drugs for treatment of the Alzheimer disease and as photo- or radiosensitizers in cancer therapy [1].
Small molecules bind to the DNA and HSA in different...
Mr
Marcin Sterniczuk
(Institiute of Nuclear Chemistry and Technology)
16/10/2012, 16:01
Other
poster preferred
Radicals play an important role in many radiolytic and catalytic reactions. However, their structure and properties are difficult to study because of extremely high reactivity. Matrix isolation technique in noble gas matrices and trapping in zeolites are the methods of choice for studying electronic structure and geometry of radical ions in solids.
We present the study of paramagnetic carbon...
Mr
Alexander Arndt
(Physikalisch-Technische Bundesanstalt (PTB))
16/10/2012, 16:02
Early Biophysical Processes
poster preferred
The advent of heavy ions for cancer therapy has emphasised the need for novel dosimetric concepts that ideally should also account for the biological effectiveness of radiation. One approach to obtain such new dosimetric concepts is the field of nanodosimetry, where the particle track structure is taken into account by measurement [1] or by simulation [2] of ionisation cluster size...
Malgorzata Jakubowska
(Institute of Applied Radiation Chemistry, Lodz University of Technology, Lodz, Poland)
16/10/2012, 16:03
Other
poster preferred
Glycosaminoglycans are linear polysaccharides consisting of repeating disaccharide units. Because of the presence of negatively charged sulfate and carboxyl groups they are polyelectrolytes. They occur on animal cell surface and in the extracellular matrix. Glycosaminoglycans present on endothelium cell surface allow an interaction with the underlying subendothelium and blood components, but...
Ms
Anna Konarska
(Institute of Applied Radiation Chemistry, Lodz University of Technology)
16/10/2012, 16:04
Other
poster preferred
Sanguinarine and coralyne are isoquinoline alkaloids isolated from plants. The use of these alkaloids as medicines by humans dates to several thousands of years ago and recently they are applied in cancer therapy.
Naturally occurring alkaloids are effective in cancer treatment but its mechanism of action has not been fully elucidated. Sanguinarine is reduced upon entering the cell and then...
Mr
Bartosz Michalowski
(Institute od Applied Radiation Chemistry, Lodz University of Technology, Lodz, Poland)
16/10/2012, 16:05
Other
poster preferred
Because of the fact that the oxidation product of hydroethidine (HE), 2-hydroxyethidium (2-OH-E+), is an unique, fluorescent marker for superoxide radical anion (O2โข-), hydroethidine has become one of the most used fluorogenic probes for its intracellular detection. Although, HE can be used rather as a qualitative than quantitative probe for detection of superoxide, it is still worthwhile to...
Dr
Elizabeth Ainsbury
(Health Protection Agency)
16/10/2012, 16:06
Biological and Physical Dosimetry
poster preferred
The classical methods for dose estimation in radiation cytogenetics are now extremely well defined. However, classical statistical methodology requires that chromosome aberration yields are considered as something โfixed.โ This 'frequentist' approach takes into account only the chromosomal damage which has been identified in a particular sample, and as such does not fully consider the...
Dr
Ulrike Kulka
(German Federal Office for Radiation Protection)
16/10/2012, 16:07
Biological and Physical Dosimetry
poster preferred
Creating a sustainable network in biological and retrospective dosimetry that involves a large number of experienced laboratories throughout the European Union (EU) will significantly improve the accident and emergency response capabilities in case of a large-scale radiological emergency. A well organised cooperative action involving EU laboratories will offer the only chance for a fast and...
Prof.
Anna Lankoff
(Institute of Nuclear Chemistry and Technology, Centre for Radiobiology and Biological Dosimetry, Warsaw, Poland; Jan Kochanowski University, Dept. of Radiobiology and Immunology, Kielce, Poland)
16/10/2012, 16:08
Biological and Physical Dosimetry
poster preferred
Dicentric assay is currently a gold standard for classic biodosimetry because it possess all features of an ideal assay: low background level, clear dose-effect relationship for different radiation qualities and dose rates, specificity to ionizing radiation, low invasiveness, good reproducibility and comparability of in vitro and in vivo results. This method is based on the assumption that the...
Dr
Octรกvia Monteiro Gil
(Instituto Tecnolรณgico e Nuclear, Instituto Superior Tรฉcnico, Universidade Tรฉcnica de Lisboa)
16/10/2012, 16:09
Biological and Physical Dosimetry
poster preferred
The main aim of a radiation study is to assess the risk of the population exposed to ionizing radiation. The work of the dose assessment may be very difficult because dose information about the population exposed is often indirect and incomplete. It is very important, therefore, to find a way to estimate reasonable and reliable doses of the population by a retrospective method such as...
Dr
Maria Wojewรณdzka
(Institute of Nuclear Chemistry and Technology, Centre for Radiobiology and Biological Dosimetry, Warsaw, Poland)
16/10/2012, 16:10
Biological and Physical Dosimetry
poster preferred
The scoring of ฮณ-H2AX foci as a measure for double-strand breaks (DSB) can be used
to estimate exposure to the very low doses of radiation and shows that the yield of foci induced by ionizing radiation increases linearly with the radiation dose. These findings indicate that quantitation of ฮณ-H2AX foci is a promising tool for biological dosimetry. Quantification of ฮณ-H2AX foci can be achieved...
Dr
Francisco Cutanda Henrรญquez
(Instituto de Salud Carlos III)
16/10/2012, 16:11
Biological and Physical Dosimetry
poster preferred
Modern treatment planning allows the achievement of complex goals. Minimum and maximum doses cannot be considered as adequate figures of merit for the quality of a plan, and that is the reason why a complex set of parameters has to be evaluated when an irradiation plan is to be assessed. Particularly, radiobiological indices are useful tools for this evaluation, as they relate the plan to...
Dr
Katarzyna Golnik
(Central Laboratory for Radiological Protection)
16/10/2012, 16:12
Biological and Physical Dosimetry
poster preferred
The analysis of dicentric chromosomes in human peripheral blood lymphocytes is the most reliable and accurate method for estimating a recent absorbed dose following external exposure to ionising radiation. The frequencies of dicentrics per grey vary depending on the variations in both the linear energy transfer (LET) and the relative biological effectiveness (RBE) of any particular radiation....
Dr
Hasan TUNER
(Department of Physics, Faculty of Science and Literature, University of Balikesir, Balikesir, Turkey)
16/10/2012, 16:13
Biological and Physical Dosimetry
poster preferred
Potential ESR dosimetric application of different compounds of sodium tartrate such as sodium tartrate dihydrate (NaTA), sodium bitartrate monohydrate (Na-bTA), and potassium sodium tartrate tetrahydrate (KNaTA) were investigated in the range of 0.5 โ 50 Gy. Comparisons were also made with alanine (AL) [1], ammonium tartrate (AmTA) [2] and lithium formate (LiF) [3] samples. It is found that...
Dr
Mariagabriella Pugliese
(Dipartimento di Scienze Fisiche, Universitร degli Studi di Napoli Federico II, Napoli, Italy and INFN Sezione di Napoli)
16/10/2012, 16:14
Biological and Physical Dosimetry
poster preferred
Ionizing radiation from natural sources such as cosmic rays and terrestrial radiation, is the major contribute to the exposure for public population. In particular gamma radiation is the largest contributor to the external dose of the humans. Today it is assessed that the majority of inhabitants in urban areas spends about 80% of their time indoor, so their exposure to ionizing radiation from...
Ms
najmeh assadi
(babol university of medical sciences)
16/10/2012, 16:15
Biological and Physical Dosimetry
poster preferred
During humans lifetime,they are exposed to physical, chemical, and biological agents. Ionizing radiation as a ubiquitous environmental physical agent induces DNA damage directly (as a result of deposition of energy in cells) or indirectly (as a result of free radical formation and oxidative damage . And because of Radiation usage in the treatment of a wide range of malignancies. Therefore,...
Dr
Amilcar Lopes Antonio
(1-School of Agriculture, Polytechnic Institute of Braganรงa, Portugal. 2-Department of Fundamental Physics, University of Salamanca, Spain. 3-IST/ITN, Nuclear and Technological Institute, Portugal)
16/10/2012, 16:16
Biological and Physical Dosimetry
poster preferred
Food irradiation is a well established process, approved by international organizations of food (FAO โ Food and Agriculture Organization) and health (WHO โ World Health Organization), and regulated in European Union by the Directive 1999/2/EC.
Chestnuts are a seasonal fruit that must be post-harvest treated to meet the food safety regulations. Until recently, this was done with methyl bromide...
Dr
Ruth Wilkins
(Health Canada)
16/10/2012, 16:17
Biological and Physical Dosimetry
poster preferred
In the event of a large scale radiological/nuclear emergency, biological dosimetry is essential for providing timely assessments of radiation exposure for the general population and to identify first responders who must be restricted from further exposure. The dicentric chromosome assay (DCA) is currently the accepted biodosimetry method for radiation dose assessment, however in a mass...
Mr
Ryo Akima
(Department of Diagnostic Radiology & Radiation Oncology, Kochi Medical School)
16/10/2012, 16:18
Biological and Physical Dosimetry
poster preferred
Purpose: For patients who experience radiation dermatitis during radiotherapy, we sometimes use pharmaceutical agents applied topically, such as creams and lotions. Irradiation of treated parts thus sometimes occurs. If a substance exists in the skin surface, the skin dose might be slightly affected by such exposure, and the agent could become a factor associated with radiation injury. The...
Mrs
Natalia Sotnik
(Southern Urals Biophysics Institute)
16/10/2012, 16:19
Biological and Physical Dosimetry
poster preferred
Objective: Assessment of structural genome damages in Mayak workers exposed occupationally to prolonged external gamma-radiation and/or internal alpha-radiation using mFISH.
Material and Methods: Slides of 64 workers were analyzed in the study. Average age of the included individuals was 73.3ยฑ0.9 years. Workers included in the study were exposed occupationally to prolonged external...
Ms
Alexandra Vozilova
(Russian Federation)
16/10/2012, 16:20
Biological and Physical Dosimetry
poster preferred
Population exposure to ionizing radiation in the Urals region (Russia) occurred as a result of failures in the technological processes at the Mayak plutonium facility in early 1950s. A major source of environmental contamination was releases of liquid radioactive wastes into the Techa River in 1949โ1956 with peak amounts from September 1950 until November 1951. Over 30,000 persons living in...
Dr
Sergey Osovets
(Southern Urals Biophysics Institute)
16/10/2012, 16:21
Biological and Physical Dosimetry
poster preferred
In spite of the great variety of bioindication and biodosimetry methods, World Health Organisation (WHO) and International Atomic Energy Agency (IAEA) accept dose reconstructions by cytogenetic methods based on the analysis of specific chromosomal aberrations (DIC, FISH techniques) on metaphases from peripheral blood cells. Capabilities and characteristics of cytogenetic indication and...
Ms
Lenka Zรกrybnickรก
(Faculty of Military health Sciences, University of Defence)
16/10/2012, 16:22
Biological and Physical Dosimetry
poster preferred
Natural killer cells (NK cells) belong to white blood cells which care for anti-tumour and anti-viral control in organism. They can be distinguished from other lymphocyte subpopulations by specific surface markers detection. Our study is aimed at a sensitivity of NK cells to ionizing gamma irradiation. Generally, lymphocytes induce cell apoptosis in a consequence of gamma irradiation....
Dr
Ralf Kriehuber
(Radiation Biology Group, Department of Safety and Radiation Protection, Forschungszentrum Jรผlich, D-52425 Jรผlich, Germany)
16/10/2012, 16:23
Biological and Physical Dosimetry
poster preferred
In case of a large-scale radiation accident with involvement of individuals without physical dosimeters it is important to identify individuals who have received a moderate to high radiation dose to ensure proper medical care. As current methods are time-consuming, a fast and reliable method based on gene expression alterations is developed.
Human blood of 3 male and 3 female healthy donors,...
Dr
Akira Furukawa
(National Institute of Radiological Sciences (Japan))
16/10/2012, 16:24
Biological and Physical Dosimetry
poster preferred
Metaphase finder is a tool for biodosimetry that finds metaphase chromosomes among blood cells on slide glasses. It consists of microscope, auto-focus system, automated X-Y stage, camera and computer. It does the image diagnosis of the microscopic images of the slide glasses, and displays the positions of metaphase cells. A metaphase finder is used for the personnel worked at Fukushima nuclear...
Dr
ADRIANO M. SANTOS
(Nuclear Technology Development Center - National Commission on Nuclear Energy)
16/10/2012, 16:25
Biological and Physical Dosimetry
poster preferred
Different therapeutic radiopharmaceuticals containing the P-32, Re-188, Sr-89, and Y-90 radioisotopes have been used effectively in alleviating bone pain resulting from metastases, synovectomy, and tumors. These radioisotopes all present the ability to emit high doses of beta radiation, leading to the death of tumor cells. Similarly, the Gd-159 isotope emits negative beta (1001 keV) and gamma...
Dr
Kamil Brzoska
(Institute of Nuclear Chemistry and Technology, Warsaw, Poland)
16/10/2012, 16:26
Biological and Physical Dosimetry
poster preferred
The strategic research project entitled โTechnologies Supporting Development of Safe Nuclear Power Engineeringโ is a response to the demand for the Polandโs energetic safety optimization in the light of nuclear power engineering introduction. The strategic project will link research performed by Polish research teams with that which is carried out abroad and provide Polish scientists and...
Dr
Paola Fattibene
(Department of Technology and Health, Istituto Superiore di Sanitร , Rome, Italy)
16/10/2012, 16:27
Biological and Physical Dosimetry
poster preferred
In the retrospective dose assessment of individuals potentially exposed to ionizing radiation after an accident, dosimetry with inert materials can complement or be used as an alternative to biodosimetry assays.
In the framework of the EU funded project Multibiodose (www.MULTIBIODOSE.eu), it is proposed to implement a combined system of dose assessment based on the use of EPR and OSL...
Dr
Katarzyna Majchrzak
(National Centre for Radiation Protection in Health Care, Lodz, Poland)
16/10/2012, 16:28
Biological and Physical Dosimetry
poster preferred
Introduction:
In 2011 National Centre for Radiation Protection in Health Care (NCRPHC) together with sanitary inspections accomplished the pilot project for estimation of the entrance surface dose (ESD) for the most frequent plane film X-ray examinations. As a result the compliance with diagnostic reference levels (DRLs) in routine practice of the supervised departments were...
G Jones
(University of Leicester)
16/10/2012, 16:29
DNA Damage and Repair
poster preferred
Bladder cancer patients suffer significant rates of treatment failure, including high rates of recurrence and poor outcomes for advanced disease. If mechanisms to improve tumour cell treatment sensitivity could be identified and/or if patient tumour response could be predicted, it should be possible to improve both control and survival rates, i.e. by selecting the most appropriate treatment...
Dr
Jun-ichi Asakawa
(Dept. of Genetics, Radiation Effects Research Foundation)
16/10/2012, 16:30
Other
poster preferred
We used two methods to estimate genetic effects of radiation. The first method was the comparative genomic hybridization (CGH) with high-density microarray containing 2.1 million probes (HD-2 microarray), which permits high-resolution examination of copy number changes (CNV: deletions and amplifications) throughout the whole genomes between two DNA samples. Refinement of the experimental and...
Mrs
Ursula Oestreicher
(Bundesamt fรผr Strahlenschutz)
16/10/2012, 16:31
DNA Damage and Repair
poster preferred
The increasing use of paediatric computed tomography (CT) worldwide has raised the question of possible late effects from exposure to ionising radiation. The European collaborative EPI-CT project aims at studying the cancer risks and the underlying biological effects in an international cohort study. The project is coordinated by the Section of Environment and Radiation at the International...
Dr
CHA SOON KIM
(Radiation Health Research Institute, KHNP)
16/10/2012, 16:32
DNA Damage and Repair
poster preferred
H2AX plays an important role in chromatin reorganization implicated in DNA repair and apoptosis under various DNA damaging conditions. In this study, the interaction between TOPORS and H2AX was verified in vitro and in vivo using mammalian cell extracts exposed to diverse DNA damaging stresses such as ionizing radiation, doxorubicin, camptothecin, and hydrogen peroxide. In vitro assays for...
Dr
Ghazi Alsbeih
(King Faisal Specialist Hospital & Research Centre)
16/10/2012, 16:33
DNA Damage and Repair
poster preferred
DNA double-strand breaks (DSBs) in irradiated cells are primarily responsible for the biological effects of ionizing radiation. The restitution of the broken ends depends on repair potential of the cells and determines their fate: survival, transformation or death. It has been suggested that differences in DSBs repair capacity between individuals and cultured cell strains may explain the...
Judith Seel
(Universitรคt der Bundeswehr Mรผnchen)
16/10/2012, 16:34
DNA Damage and Repair
poster preferred
High LET irradiation of living cells using heavy ions generates a high amount of DNA double strand breaks (DSB) in close vicinity to each other along the ion track. Various repair proteins cluster to the damage sites, e.g. ฮณH2AX and 53BP1, and form repair foci of a gross size of about 1 ยตm. Due to the fact that one focus covers more than one DSB, a fine structure within the focus can be...
Zuzana Sinkorova
(University of Defence)
16/10/2012, 16:35
DNA Damage and Repair
poster preferred
Whole body ionizing gamma irradiation represents genotoxic stress, which has a decisive influence on all blood cell lines. Lymphocytes belong to the most radiosensitive cell lineages. After exposure of the organism to ionizing irradiation they die by apoptosis, the extent of which depends on the reeived dose. Bone marrow contains a reserve pool of functionally mature granulocytes, which might...
Dr
Justyna Miszczyk
(Institute of Nuclear Physics PAN, Krakรณw, Poland)
16/10/2012, 16:36
Biological and Physical Dosimetry
poster preferred
Colorectal cancer is one of the most commonly occurring malignancies in both men and women. However, application of radiotherapy might be beneficial depending on individual susceptibility or even may cause risk of secondary cancers and others complications. Therefore, studies on influence of endogenic or egzogenic factors on the bodyโs response in modeling a therapeutic procedure are still...
Nicole Averbeck
(GSI Helmholtzzentrum fรผr Schwerionenforschung)
16/10/2012, 16:37
DNA Damage and Repair
poster preferred
We study DNA double strand break (DSB) repair with heavy ion radiation. As densely ionizing radiation it generates strictly localized DSBs within the nucleus and thus, represents an excellent tool for investigating the recruitment of repair factors to DSBs by immunofluorescence. Applying this technique we were able to show that with increasing lesion complexity DSBs induced in the G1 cell...
Ms
Alyona Pogodina
(Ural Research Centre for Radiation Medicine)
16/10/2012, 16:38
DNA Damage and Repair
poster preferred
Our purpose was investigation DNA single-strand breaks (SSB) and double-strand breaks (DSB) levels in peripheral blood lymphocytes exposed individuals 60 years after exposure.
Two groups of humans was formed: exposed (51 subjects) and non-exposed (24 subjects). In the group of exposed individuals were identified individuals with chronicradiation syndrome (CRS) โ 8 individuals, and with...
Dr
Otilia Nuta
(Radiation and Environmental Science Centre, DIT, Republic of Ireland; Department of Toxicogenetics, Leiden University Medical Center, the Netherlands; Health Protection Agency, Centre for Radiation, Chemical and Environmental Hazards, Oxfordshire, U.K.)
16/10/2012, 16:39
DNA Damage and Repair
poster preferred
There have been suggestions that the telomerase system is involved in radiation induced genomic instability.
The aim of this study was to investigate the involvement of telomerase in the development of chromosomal damage and its repair at different stages of the cell cycle following an exposure of human fibroblasts to ionizing radiation.
We examine the relationship between several...
Mindaugas Malcius
(Center for Physical Sciences and Technology, Vilnius, Lithuania)
16/10/2012, 16:40
DNA Damage and Repair
poster preferred
In this investigation pancreatic cancer cells MIA PaCa-2 were irradiated with various doses
of proton radiation: from 1 Gy to 30 Gy. Experiments were done using Tandetron 4110 accelerator
in Center for Physical Sciences and Technology(which is in Vilnius, Lithuania). Irradiation was
executed when cells formed monolayer. Distance from exit window to sample was 22 mm.
Diameter of proton exit...
Dr
Sandro Conrad
(Technical University of Darmstadt)
16/10/2012, 16:41
DNA Damage and Repair
poster preferred
The major repair pathways for DNA double-strand breaks (DSBs) are non-homologous end-joining (NHEJ) and homologous recombination (HR). NHEJ is active in all cell cycle phases and repairs DSBs with fast kinetics. It represents the major repair pathway in G1 and G2. In contrast, HR is the main repair pathway for replication-associated DSBs in S phase and also repairs a subfraction of DSBs in G2....
Dr
Georgia Terzoudi
(Radiobiology Laboratory, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, National Centre for Scientific Research โDemokritosโ Athens, Greece)
16/10/2012, 16:42
DNA Damage and Repair
poster preferred
Radiation can increase significantly the level of oxidative stress that cell have to sustain and mitigate. Persistent of such stress can lead to unrepaired or misrepaired DNA lesions the accumulation of which is considered for many the initiation point for chromosomal instability, neoplastic transformation and human pathogenesis. Indeed, the presence of high oxidative stress in human patients...
Dr
Gabriel Pantelias
(Radiobiology Laboratory, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, National Centre for Scientific Research โDemokritosโ Athens, Greece)
16/10/2012, 16:43
DNA Damage and Repair
poster preferred
The formation of diverse chromosomal aberrations following irradiation at different cell-cycle stages remain a long standing controversy, probably because most of the studies have focused on elucidating the enzymatic repair mechanisms involved using simple DNA substrates. Yet, recognition, processing and repair of DNA damage occur within the nucleoprotein complex of chromatin which is dynamic...
