246.
Investigating DNA repair centers in mammalian cells and their impact on dose-response linearity
Dr
Sylvain V. Costes
(Lawrence Berkeley National Laboratory, Berkeley, USA)
16/10/2012, 13:30
DNA Damage and Repair
oral (invited speaker)
We recently showed in non-malignant human breast cells (MCF10A) that at doses of X-ray above or equal to 1 Gy, radiation-induced foci (RIF) do not increase linearly with dose. RIFs were characterized by the local recruitment of DNA damage sensing proteins p53 binding protein (53BP1). Using a mathematical model of RIF kinetics we also showed that RIF induction rate increased with increasing...
Dr
Dik C. van Gent
(Erasmus MC)
16/10/2012, 14:00
DNA Damage and Repair
oral (invited speaker)
The effects of low and protracted doses of ionizing radiation on humans are only partially understood. The shape of the dose response curve at low and protracted doses cannot be measured accurately using standard epidemiological methods. However, this information is needed for rationale policy decisions for setting exposure limits. Therefore, we set out to determine a gene expression signature...
Prof.
Andreyan Osipov
(Burnazyan Federal Medical Biophysical Center of the Federal Medical-Biological Agency)
16/10/2012, 14:30
DNA Damage and Repair
oral (15 minutes)
There is a considerable controversy as to whether DNA damage induced by low doses and low dose rates of ionizing radiation is treated similarly by cellular defence machineries and what downstream delayed effects it causes compared to moderate to high doses. This constitutes the major challenge for the linear no-threshold model (LNT) currently used for radiological risk estimates. Among various...
Dr
Dianov Grigory
(University of Oxford)
16/10/2012, 14:45
DNA Damage and Repair
oral (15 minutes)
The deubiquitylation enzyme USP7 (also known as HAUSP) plays a major role in regulating genome stability and cancer prevention by controlling the key proteins involved in the cellular DNA damage response, including Mdm2, p53, FOXO4 and PTEN. Deletion of USP7 leads to early embryonic lethality mainly due to increased p53 levels and cell proliferation arrest. Despite such an important role in...
Mrs
Elina Staaf
(Centre for Radiation Protection Research, GMT department, Stockholm University, Sweden)
16/10/2012, 15:00
DNA Damage and Repair
oral (15 minutes)
Increasing exposure of cancer patients to a mixed beam of high and low linear energy transfer (LET) ionizing radiation is an issue of growing concern. Little is known about the health effects of exposing organisms and cells to mixed beam irradiation. The effect of combined exposures has mainly been assessed with clonogenic survival or cytogenetic methods, and the results are contradictory. The...
Dr
Dorothee Deckbar
(Darmstadt University of Technology, Radiation Biology and DNA Repair)
16/10/2012, 15:15
DNA Damage and Repair
oral (15 minutes)
The DNA damage response pathways involve processes of double-strand break (DSB) repair and cell cycle checkpoint control. The coordinated interplay of these mechanisms is crucial for the maintenance of the genomic integrity as the progression through critical cell cycle phases with unrepaired DSBs can promote chromosome aberration formation.
We recently demonstrated that the G1/S and G2/M...
