Speaker
Dr
Ainars Bajinskis
(Stockholm University)
Description
In the last decade studies have shown that unique gene and protein expressions are induced in response to low doses (in the range of 10 – 100 mGy) compared to high doses (1 to 4 Gy) of ionising radiation. Studies showed that different numbers of genes or proteins are altered in response to radiation and only a small number of genes or proteins were the same for low and high doses.
We aimed to investigate this in more details by irradiating cells with two different doses in the low dose region and also study if the radical scavenger DMSO could modify the protein response. CHO AA8 cells were exposed to acute doses of 10, 100 or 1000 mGy γ-rays (dose rate 0.43 Gy/min) or α-particles in the absence or presence of DMSO. The proteins were extracted from cells 3 hours after irradiation and used for protein expression analysis by two-dimensional polyacrylamide gel electrophoresis.
The results presented demonstrate that unique patterns of up or down regulated protein spots are produced in response to different radiation qualities as well as doses. Work is now in progress to identify the proteins involved and to provide a better understanding of the cellular processes induced by low doses of radiation from high or low LET radiation.
Primary author
Dr
Ainars Bajinskis
(Stockholm University)
Co-author
Prof.
Mats Harms-Ringdahl
(Stockholm University)
