Speaker
Mrs
Arundhathi Sriharshan
(Helmholtz Center Munich)
Description
Epidemiological data show that ionising radiation increases the risk of cardiovascular disease. The endothelium is one of the main targets of radiation-induced damage. Rapid radiation-induced alterations in the biological processes were investigated after exposure to a clinically relevant radiation dose (2.5 Gy gamma radiation). The changes in protein expression were determined using the human endothelial cell line EA.hy926 as a model.
Two complementary proteomic approaches, SILAC (Stable Isotope Labelling with Amino acids in Cell culture) and 2D-DIGE (Two Dimensional Difference-in-Gel-Electrophoresis) were used. The proteomes of the endothelial cells were analysed 4 h and 24 h after irradiation. Differentially expressed proteins were identified and quantified by MALDI-TOF/TOF and LTQ Orbitrap tandem mass spectrometry.
The deregulated proteins were mainly categorised in four key pathways: (i) glycolysis/ gluconeogenesis and synthesis/degradation of ketone bodies, (ii) oxidative phosphorylation, (iii) Rho-mediated cell motility and (iv) non-homologous end joining. We suggest that these alterations facilitate the repair processes needed to overcome the stress caused by irradiation and are indicative of the vascular damage leading to radiation-induced cardio- and cerebrovascular impairment.
Primary author
Mrs
Arundhathi Sriharshan
(Helmholtz Center Munich)
Co-authors
Dr
Hakan Sarioglu
(Helmholtz Center Munich)
Prof.
Horst Zitzelsberger
(Helmholtz Center Munich)
Dr
Karsten Boldt
(University of Tuebingen)
Dr
Ludwig Hieber
(Helmholtz Center Munich)
Prof.
Marius Ueffing
(Helmholtz Center Munich)
Prof.
Michael. J. Atkinson
(Helmholtz Center Munich)
Dr
Omid Azimzadeh
(Helmholtz Center Munich)
Dr
Soile Tapio
(Helmholtz Center Munich)
Dr
Zarko Barjaktarovic
(Helmholtz Center Munich)
