Fourier transform infrared (FTIR) (micro-)spectroscopy is a label-free and non-destructive vibrational tool that has been successfully applied to study not only the amyloid structural properties and aggregation mechanisms directly in cells, tissues, or biofluids, but also to gain new insights into the mechanisms of amyloid formation and toxicity[1]. In particular, the use of an infrared...
Ultrafast multidimensional IR spectroscopy (2D-IR) can provide detailed information on local structural dynamics in peptide, proteins or complex systems like models of protein condensates. 2D-IR is a femtosecond laser spectroscopy with its intrinsic time-resolution on par with the fundamental timescale of chemical dynamics, fs to ps. For biological systems the capability to directly probe...
