Using X-ray diffraction for structure determination is further complicated when the irradiated particles have random and unknown orientations, because it means that the relations between the diffraction images are also unknown. Algorithms exist for recovering the relative orientations between diffraction patterns, but they do not always converge, especially not when faced with scarce or noisy...
MS SPIDOC is an innovative sample delivery system tailored for single-particle imaging at X-ray Free-Electron Lasers (XFELs) and adaptable to most large-scale facility beamlines. It accommodates a wide range of biological samples, from small proteins to megadalton (MDa) complexes. Utilizing nano-electrospray ionization, ionic samples are $m/z$-filtered and structurally separated before...
Non-aromatic fluorescence in biomolecules represents a fascinating photophysical phenomenon that challenges conventional understanding of fluorescent mechanisms.. This presentation outlines our ongoing investigation using a combined approach of time-resolved X-ray absorption spectroscopy (TrXAS) and multiple vibrational spectroscopic techniques to explore the fundamental processes governing...
Normal-to-cancer transition (NTC) is still an ill-understood process, closely associated to cellular biomechanical properties. These are strongly dependent on intracellular water´s structural and dynamical profiles, which play a fundamental role in cellular function. Improved chemotherapeutic strategies are an urgent clinical need, since cancer is still the second leading cause of death...
Tip-enhanced Raman scattering (TERS) has emerged as a powerful technique for exploring biomaterials at nanometer resolution. It provides chemically specific, label-free imaging beyond the optical diffraction limit. It is especially advantageous when labels cannot be used, a main requirement for super-resolution fluorescence methods, which rely on fluorescent labeling. TERS provides direct...
Studying structural changes associated with protein aggregation is challenging and often requires a combination of experimental techniques to capture insights at the molecular level across different scales, from nanometers to microns. Studying this process becomes even more complex when aggregation occurs in the presence of molecular co-factors, nucleic acids among them, and when the resulting...
