The study and characterization of Cultural Heritage (CH) materials requires the application and the development of innovative technologies, also related to accelerators, and fine-tuning preparation procedures of samples and, moreover, the requirements of noninvasiveness and minimal interaction with the artworks must be satisfied. In this sense, the INFN-CHNet, the network dedicated to the study of CH materials, monitored by the National Technology Transfer Committee of INFN, encounters these needs by developing innovative instrumentations and by collaborating with other professionals in the Cultural Heritage field for data interpretation. The INFN-CHNet nodes, DAΦNE-Light and LASR3 laboratories, will present the technologies developed and experiments performed on an unknown version of the painting “Lo Spasimo di Palermo” by Raffaello Sanzio. Preliminary results will be discussed also from the art-historical point of view, in a synergic collaboration between scientific and art history research.