Brillouin microscopy for cell and tissue biomechanical imaging

by Giuliano Scarcelli (University of Maryland)

Thursday 16 Dec 2021, 14:00 → 16:00 Europe/Rome

Edificio E. Fermi - Aula 5 (Dip. di Fisica)

The interaction between photons and acoustic phonons within materials, first described by Leon Brillouin, has been widely investigated to characterize mechanical and physical properties of samples. To translate this technology to biomedical applications where mechanical properties are often critical, our lab has developed high-resolution spectrometers at high throughput and combined them with optical microscopes to yield 3D imaging modalities that use label-free biophysical properties as contrast mechanisms for imaging. More recently we have demonstrated increased Brillouin microscopy resolution to characterize intracellular modulus and we developed a stiffness based flow cytometry platform.