Multimodal imaging of single cells with high performance analytical techniques: recents results with infrared synchrotron radiation

Europe/Rome
Puls Room (Laboratori Nazionali di Frascati, Via Enrico Fermi 40, Frascati)

Puls Room

Laboratori Nazionali di Frascati, Via Enrico Fermi 40, Frascati

Via Enrico Fermi 40 00044 Frascati
Description
ABSTRACT

Molecular/elemental imaging of individual cells with a lateral resolution below 1x1 µm is the current technological challenge for investigating the smallest dimension of living systems. In the race for such analytical performances, several techniques have been successfully developed; some are using probes for determining given cellular contents, others use interaction between cellular matter with light or elements for contents characterization. Morphological techniques may also provide information about dimensions of a cell, thus opening the way to quantitative studies when combined to other techniques. Here is presented a prospective view about the methodological requirements for implementing a multimodal imaging of an individual cell, notably combining FTIR imaging coupled to synchrotron radiation source and Brewster-angle microscopy, but later also using the Pulsed-Raman and X-Ray fluorescence microscopy techniques. Expected opportunities for cell biology investigations will be discussed in face of information every technique is able to provide.

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