17-19 October 2018
Laboratori Nazionali di Frascati INFN
Europe/Rome timezone

XAS measurements of high-diluted Cu-Amylin complexes

18 Oct 2018, 18:00
30m
Aula Salvini (Laboratori Nazionali di Frascati INFN)

Aula Salvini

Laboratori Nazionali di Frascati INFN

Via E. Fermi 40 00044 Frascati (RM)

Speaker

Emiliano De Santis (ROMA2)

Description

The amyloidogenic islet amyloid polypeptide (IAPP) and the associated pro-peptide ProIAPP1-48 are involved in cell death in type 2 diabetes mellitus. It has been observed that interactions of this peptide with metal ions are connected with both the cytotoxicity of the peptides and their deposition as amyloids fibrils associated. In particular, Cu(II) seems to inhibit amyloid fibril formation, thus suggesting that Cu homeostasis unbalance may be involved in the type 2 diabetes mellitus pathogenesis. We performed X-ray Absorption Spectroscopy (XAS) measurements of Cu(II)-amylin complexes in physiological concentration conditions, namely at a very low concentration of absorbing atoms, i.e. 10 μM of Cu(II) ions. Such low concentrations have been faced by exploiting the High Energy Resolved Fluorescence Detection (HERFD) XAS, as implemented at the ESRF beamline BM16 (FAME-UHD). Our results demonstrated the feasibility of XAS measurements on ultra-diluted biological samples, thus making possible experiments very close to the physiological conditions.

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