New diagnosis and therapy procedures by utilizing advanced optical multi-contrast and multi-parameter spectroscopy

by Juergen Popp (Leibniz Institute of Photonic Technology; Institute of Physical Chemistry & Abbe-Center of Photonics, FS University Jena Germany)

Friday 17 Feb 2017, 15:00 → 16:00 Europe/Rome

Aula Amaldi (Dip. di Fisica - Edificio G. Marconi)

To better understand, treat or even cure diseases, an urging demand for new diagnostic methods providing a differential diagnosis and allow for accurate and early treatment exits. In the past few years, biophotonics has witnessed the development of methods and technologies that are potentially in a position to meet these challenges. In this regard, spectroscopic methods offer the great advantage of obtaining molecular information directly from the examined sample (e.g. cells, tissue) in a label-free manner to provide a clinician with adequate support in the form of clinically-relevant information under both ex vivo and in vivo conditions. One particularly efficient method in this regard is Raman spectroscopy to monitor intrinsic molecular vibrations distinct for every molecule. Within this contribution we will show, that Raman spectroscopy holds great promise as point-of-care approach for a fast identification of pathogens and the determination of their antibiotic resistances, which is crucial for patient’s survival. Furthermore, it will be shown that the combination of Raman approaches with other spectroscopic technologies provides a sensitive and selective diagnostic tool to potentially solve challenges currently faced by clinical pathology. We will among others introduce a combined Raman /FLIM (fluorescence lifetime imaging microscopy) fiber optical probe for in- vivo tissue screening. Furthermore, we demonstrate how the combination of CARS (coherent anti-Stokes Raman scattering), SHG (second harmonic generation) and two-photon excited autofluorescence (TPEF) enables the characterization of the morphochemistry of frozen section biopsy specimens. Note: Abbiamo controllato la disponibilità aule e al momento al Vecchio Edificio non risultano libere aule per le ore 15 di venerdì 17 (la Conversi risulta prenotata per evento da L.Leuzzi e l'aula Careri è libera dalle 16). Contiamo di contattare i responsabili della lezione in Careri per chiedere uno scambio aula. Pensiamo che un seminario alle 16 di Venerdì possa raccogliere meno adesioni.